François Delori, Ph.D.

Research Story

Dr. Delori’s field of expertise is the examination of the retina by noninvasive means. By shining various colors of light onto the retina and analyzing the nature of the light reflected by the retina, one can obtain quantitative information about many important biological parameters –  such as oxygen levels in retinal blood vessels, speed of blood flow, diffusion of nutrients, and quantities of various pigments. (Pigments are colored substances that can be studied because they absorb light.) Dr. Delori has pioneered novel imaging techniques of the retina and has developed advanced optical techniques to study the role of lipofuscin and melanin pigments in the retinal pigmented epithelium as well as new ways to measure the distribution of macular pigment in the neural retina (see diagram). These techniques are now used world-wide for research in the field of aging and retinal degenerations, as well as for tools to diagnose disease.

Lipofuscin, melanin, and macular pigment are three pigments that may play important roles in the causation of AMD. The ability to measure these pigments may not only provide new basic information on AMD and other retinal degenerations, but may also allow novel diagnostic tests to detect early disease and monitor the effects of drug intervention.

A look at the center of vision: the macula

delori_fig.jpgThe macula is the region of the eye devoted to precise vision. Its center, the fovea, is about as large as the head of a pin (0.3 mm across) and contains about 115,000 cone photoreceptors cells, allowing for the detection of color and fine details. The image of two or three letters of this text on your retina occupies the entire fovea. Upon absorption of light, the photopigment (yet another type of pigment, typified by rhodopsin) in the photoreceptor cells undergoes chemical changes that produce electrical signals. These signals are conducted to the brain, where the image is interpreted. This activity is very demanding in energy and nutrients -- photoreceptor cells have the highest metabolic rate of cells in the body -- and is possible only through an exquisitely organized support and protection system.

The layer of retinal pigmented epithelium (RPE) cells is the rear lining of the retina, and is the main player in sustaining the health of the retinal photoreceptors. These cells transport nutrients for the photoreceptors from the choroid (a vascular layer that acts as source of oxygen for the deeper layers of the retina), and continuously renew the photopigment. As its name implies, the RPE also contains melanin, a very dark pigment. This pigment absorbs light reflected by the deeper retinal layers, thereby reducing unwanted light to the photoreceptors; melanin
also protects the RPE by absorbing radiation and trapping noxious chemicals.

The photoreceptor/RPE system is additionally protected by the yellow macular pigment (composed of a mixture of carotenoids – lutein and zeaxanthin). This pigment is distributed in the cones of the retina. It absorbs blue light and thereby may help reduce photochemical damage in the photoreceptors and RPE. Furthermore, macular pigment has marked anti-oxidant properties, further reducing the risk of such damage. Macular pigment is not synthetized in the body but is acquired exclusively from the diet (Spinach, broccoli, corn, and eggs are good dietary sources). Low amounts of macular pigment could result in increased photochemical damage to the photoreceptors and the RPE. Studies have shown that a low amount of carotenoids in the blood is related to increased severity of AMD. Thus, appropriate diets or supplementation is highly indicated for patients with AMD. Non-invasive methods to monitor changes in macular pigment in the retina will be critical to monitor the sucess of carotenoid therapy.

A technique to quantify this pigment was introduced by Dr. Delori, and has been adopted by many researchers. In recent experimental work in his own lab, Dr. Delori has shown that the distribution of  macular pigment is highly variable among individuals and that its pattern of distribution depends upon age and gender.

Note that the RPE is separated from the choroid a by a thin layer of collagenous connective tissue called Bruch's membrane, which acts as a mechanical support for the RPE and photoreceptors. Because oxygen and nutrients diffuse across this membrane, its integrity is critical for the well-being of the photoreceptors and the RPE.

The high metabolic activity of the photoreceptors does not occur without damage. Their environment is rich in oxygen and light, causing continuous photochemical damage to the receptors. Therefore, photoreceptors are continuously renewed; the outer part of the receptor is chopped off and digested in the RPE. The resulting waste products diffuse through Bruch's membrane to the choroidal blood vessels. However, this process is not perfect; a minute fraction of the discarded photoreceptor material remains trapped in the RPE, where is accumulates as lipofuscin pigment (also called the “age-pigment”). This process is continuous (each photoreceptor is completely renewed in about ten days), and the lipofuscin accumulates throughout life. In old age, it may occupy a large part of the RPE cell and may compromise the normal activity of the cell.

Lipofuscin can be measured by means of its fluorescence. Dr. Delori introduced noninvasive techniques to quantify lipofuscin in the living eye. Studies using these techniques have shown that the amount of lipofuscin in the RPE is highly variable in old eyes, and that the rate of accumulation of lipofuscin is affected by genetic and environmental factors. (Smoking, for example, significantly increases lipofuscin accumulation). In AMD, the levels of lipofuscin are highly variable and studies are currently aimed at understanding the progression of the pathology. Efforts by other laboratories are currently aimed at developing drugs that would decrease the accumulation of lipofuscin in the RPE. Quantification of lipofuscin by non-invasive means will be critical to assess the efficiency of these drugs.

The cause of AMD is not known. The complexity of the photoreceptor/RPE/choroid system and the strong interdependence of its components make it difficult to isolate a single causative event that triggers the disease. Nevertheless, there is agreement among researchers that the disease is caused by some RPE dysfunction, precipitated by an inherited susceptibility and/or environmental exposure. All of the above-mentioned entities (macular pigment, lipofuscin, melanin, Bruch’s membrane, and the choroidal blood supply) may have roles in causing, exacerbating, or preventing the degeneration of the retina in AMD. Non-invasive methods are required to visualize and measure all of them.

  • Age-related increases in lipofuscin in the RPE may compromise the metabolic activity of the RPE and increase photochemical damage.
  • Because melanin tends to decrease with age (similar to the graying of hair), there may be a reduction of the protection given by RPE melanin.
  • Low macular pigment levels in the retina may predispose a retina to the damage of AMD.
  • Accumulation of extracellular materials between the RPE and Bruch’s membrane leads to deposits (called drusen) large enough to be visible by ophthalmoscopy; they represent the hallmark of the early-stage AMD. These deposits may distort the RPE and cause a breakdown in the tight junctions between the RPE cells, causing blood vessels and fluids to invade the retina.
  • Alteration in choroidal blood flow may also be important.